Anatomical Pathology is a branch of Pathology that focuses on tissue diagnosis.

When you have tissue or fluid removed (biopsy) from your GP, clinic or procedure in an operating theatre, your tissue is sent to our laboratory for analysis. This includes processing and special stains and then review by our expert Anatomical Pathologists.

This analysis detects and diagnoses cancer and other benign conditions. Our Pathologists work together with your GP, Specialist, Surgeon and Oncologists to determine the best treatment option for you.

Anatomical Pathology can be separated into Histology, Cytology and Next Generation Sequencing (NGS).

NGS allows us to know more about specific cancers and their treatment options. Using this information our expert Pathologists work with your Oncologist and Surgeon to find the best treatment for you.

Meet our Anatomical Pathologists here

To view our Anatomical Pathology request forms, please click here


Detailed Overview – Histology

SydPath’s Histology service is part of the Department of Anatomical Pathology and solid tumour Genetics. Our pathologists provide sub-speciality expertise across the full range of adult histopathology testing, and are actively involved in teaching and research. We offer a comprehensive hospital based approach with a focus on collaborative care for our patients. SydPath Pathologists actively work with you to offer best care options for your patient.

Testing includes:

  • Routine histology processing and reporting of formalin fixed tissues (operative specimens, skin biopsies, endoscopic biopsies and needle core biopsies)
  • Immediate Frozen Section service with intraoperative consultation
  • Comprehensive Immunohistochemistry service with over 200 specific antibody assays run on Ventana automated platforms, including biomarker testing for HER2, BRAF, ALK and mismatch repair enzymes.
  • Skin Immunofluoresence service
  • Full Renal Biopsy service including electron microscopy and immunofluoresence
  • Paraffin In situ Hybridization Service, providing CISH testing in a variety of tumours (to view our Molecular and Advanced Pathology page please click here)

Our services are available to researchers as well as for routine diagnostic testing, including sendaway referrals from other Pathology providers.

To contact the Anatomical Pathology Department (including bookings of frozen sections) call us on (02) 8382 9217

Meet our Anatomical Pathologists here


Detailed Overview – Cytology

SydPath’s Cytopathology Service is part of the Department of Anatomical Pathology and solid tumour Genetics. Our expert cytology scientists and Cytopathologists offer a comprehensive cytology service to both St Vincent’s campus and a wide range of clients throughout the Sydney region.

Services comprise of:

Cervical screening testing (HPV):

  • HPV and Thin prep liquid based cytology

General (non gynaecological) cytology including:

  • Urines and urinary tract washings
  • Serous effusions (pleural, peritoneal, pericardial, CSF)
  • Respiratory specimens (sputum, bronchial washings & brushings)

Fine needle aspiration biopsies including:

  • Fine needle biopsies performed by pathologists on palpable lesions (same day testing provided where possible)
  • Image-guided fine needle biopsies performed by endoscopists (EBUS/EUS) or radiologists, with Rapid on-site assessment (ROSE) by scientists to advice on sample adequacy and triage for ancillary testing
  • Intra-operative/procedural fine needle biopsies (FNABs), reported by attending Pathologist on- site

To book Fine Needle Aspiration Biopsies (FNABs) call us on (02) 8382 9217. Outpatients are seen at St Vincent’s Clinic, Darlinghurst Sydney (SydPath Suite 501).

To contact the Cytology Department please call (02) 8382 9230

Meet our Anatomical Pathologists here


Detailed Overview – Next Generation Sequencing (NGS)

SydPath offers an expanding service of molecular diagnostic assays for cancer diagnosis and treatment pathways. Ranging from immunohistochemical detection of aberrantly expressed or abnormal protein (eg. MMR enzymes, HER2, ERG), in situ DNA hybridization (eg. HER2, ALK, EGFR, 1p19q, MET) and more recently, implanting Next Generation Sequencing (NGS) based detections in extracted DNA and RNA. This includes 50 to 160 gene panels.

Each tumour is different, driven by certain key mutations/genetic rearrangements, creating a “fingerprint” or profile. Next generation sequencing has revolutionised genomic studies of cancer, having facilitated the development of precision oncology treatments based on your patients ‘s tumour molecular profile. Somatic genomic testing has now become the standard of care in a variety of cancers for further diagnostic refinement, risk stratification, and therapeutic approach.

SydPath is an established NATA accredited NGS facility (since 2020). Providing standard-of-care cancer genomic diagnostics along with offering expanding research options using Thermofisher Ion Genestudio S5 and Genexus systems.

SydPath uses multiple NGS testing panels designed to investigate many relevant actionable mutations in formalin fixed paraffin embedded (FFPE) tumour samples including:

  • lung carcinoma
  • melanoma
  • colorectal carcinoma
  • and many other rare tumours

Our NGS panels cover more than 160 genes including the most common tumour driver genes. NGS also delivers high sensitivity to detect rare mutations, offering increased visibility into important driver mutations in cancer, which other approaches often miss.

SydPath’s NGS service provides a significant step forward in Personalized/Precision Medicine (PM) enabling the detection of somatic driver mutations, resistance mechanisms, quantification of mutational burden. This is a key clinical tool utilised in cancer care in St Vincent’s Hospital Sydney.

See our SydPath Next Generation Sequencing Service Guide here

Meet our Anatomical Pathologists here